بررسی اثرات آگونیست GnRH بر روی لوله های اسپرم ساز موش های صحرایی نابالغ

  T Peirouvi [1] , PhD Gh Farjah [2] , PhD RA Sadrkhanlo [3] , PhD A Ahmadi [4] D.V.MF Atabaki Pasdar [5] MSc J Behkami [6] BSc    Received: 26 Sep, 2007 Accepted: 9 July, 2008 Abstract Background & Aims: Spermatogenesis dependent to Hypophyseal Gonadotropin hormones. Secretion of Gonadotropin hormones is controlled by Hypothalamic Gonadotropin releasing hormones. GnRH analogues disregulated Hypophyseal-Gonadal axis. The aim of the present study was to evaluate the effects of GnRH-agonist administration on seminiferous tubules in immature rats. Materials & Methods: In this study the immature rats (aged 25 days) were divided into two experimental groups (n=5) and two control groups (n=5). Subcutaneous injection of GnRH-a (0.1mg/ml) and NaCl (physiologic Serum) were carried out every 24 hours for 5 days, respectively in experimental and control groups. After dissection, the testis were excised and processed for histological study 5µm serial sections. Results: The results showed that there was not significant difference in diameter of tubules between first experimental group and control groups, but there was significant difference in lumen diameter, thickness of epithelium and the number of cell layers in seminiferrous tubules.  Furthermore, there was not significant difference in diameter of tubules between second experimental group and two control groups, but it is significant in lumen diameter, thickness of epithelium and the number of cell layers (p<0.05) In addition the morphometric findings showed that there was not significant differences in diameter of seminiferrous tubules between two control groups, but the difference in lumen diameter, thickness of epithelium and number of cell layers was significant. Conclusion: We found that GnRH-agonist (buserelin) induces spermatogenesis in immature male rats.       Address : Anatomy Department Medical School, Urmia University of Medical Sciences, Nazlou Road, Urmia, Iran Tel: 0441-2770969    E-mail: [email protected]     Source: UMJ 2008: 19( 3 ): 275 ISSN: 1027-3727   [1] Assistant Professor of Anatomy, Urmia University of Medical Sciences (Corresponding Author)   [2] Assistant Professor of Anatomy, Urmia University of Medical Sciences   [3] P rofessor of Anatomical Sciences, Urmia University   [4] Resident of Anatomical Sciences, Urmia University   [5] Master of Histology , Urmia University of Medical Sciences   [6] Bachelor of Biology, Urmia University of Medical Sciences